H9908
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 | Immunofluorescence Mouse anterior prostate stained using Monoclonal Anti-phospho-Histone H3 (pSer28), clone HTA28 (Cat. No. H9908) (red) at a 1:2,000 dilution, Anti-phospho-S6 (pSer235/236) (green) and DAPI (blue). From Raleigh Kladney, Surgery Department, Washington University School of Medicine, St. Louis, MO. |
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H9908
Sigma
Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat
~0.5 mg/mL, clone HTA28, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD03457568 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 23-35 (pSer28) of human histone H3. |
| Preparation Note | Purified from culture supernatant of hybridoma cells grown in a bioreactor |
| Specificity | The antibody does not detect the unphosphorylated epitope. It detects the histone molecule at the onset of mitosis (prophase, metaphase, weaker at the beginning of anaphase, but not during late anaphase). |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | HTA28 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 15 kDa |
| species reactivity | human, bovine, hamster, mouse |
| concentration | ~0.5 mg/mL |
| application(s) | flow cytometry: suitable |
| immunoblotting: 0.5-1 μg/mL using whole extract of cultured human acute T cell leukemia Jurkat cells treated with Nocodazole. | |
| immunocytochemistry: suitable using 3.7% formaldehyde-methanol fixation | |
| microarray: suitable | |
| isotype | IgG2a |
| Gene Information | human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) |
| mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
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References
| reference | Goto, H., Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation. J. Biol. Chem. 274, 25543, (1999) Abstract |
| Hilton, M., et al., An FGF-WNT gene regulatory network controls lung mesenchyme development. Dev. Biol. 319, 426-436, (2008) Abstract | |
| Smith, A., et al., FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment. Development 134, 2895-2902, (2007) Abstract | |
| Mateescu, B., et al., Tethering of HP1 proteins to chromatin is relieved by phosphoacetylation of histone H3 EMBO Rep. 5, 490-496, (2004) Abstract |
